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![\"fig1_updated\"](\"http:\/\/127.0.0.1:49970\/equipe%20GB\/equipe%20GB\/equipe%20GB\/files\/fig1_updated.png\")
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En pr\u00e9sence d’ADP, EttA inhibe la formation de la premi\u00e8re liaison peptidique et bloque ainsi l’entr\u00e9e du complexe de d\u00e9marrage dans le cycle d’\u00e9longation de la traduction. La conformation d’EttA li\u00e9e \u00e0 l’ATP stabilise le complexe d’initiation dans une conformation favorable \u00e0 la formation de la premi\u00e8re liaison peptidique. Cette interaction stimule l’hydrolyse de l’ATP par EttA, ce qui conduit \u00e0 sa dissociation du ribosome et permet au facteur EF-G de catalyser le premier \u00e9v\u00e8nement de translocation et ainsi l’entr\u00e9e dans le cycle d’\u00e9longation. In vivo<\/em>, les cellules pour lesquelles le g\u00e8ne ettA est inactiv\u00e9, ont un d\u00e9faut d’aptitude \u00e0 survivre une longue phase stationnaire lorsqu’elles sont en co-culture avec les cellules sauvages. Il est connu que durant la phase stationnaire, le niveau d’ATP intracellulaire est faible.<\/p>\n\n\n\n <\/p>\n\n\n\n Comme la traduction de l’ARNm en prot\u00e9ine consomme plus de la moiti\u00e9 de l’\u00e9nergie cellulaire, il n’est donc pas surprenant de trouver un facteur de traduction qui r\u00e9gule ce processus. Maintenant, nous \u00e9tendons notre \u00e9tude aux 3 autres paralogues d’EttA pr\u00e9sents chez E. coli<\/em> et \u00e0 des orthologues d’EttA responsables de la r\u00e9sistance aux antibiotiques chez plusieurs bact\u00e9ries pathog\u00e8nes. Nous \u00e9tudions aussi la fonction des deux prot\u00e9ines ABC-F pr\u00e9sentes chez la cyanobact\u00e9rie Synechocystis<\/em>. Notre objectif est de cartographier les diff\u00e9rentes actions des prot\u00e9ines ABC-F sur l’appareil de traduction <\/p>\n\n\n\n <\/p>\n\n\n\n Pour ce deuxi\u00e8me projet, nous allons d\u00e9velopper une m\u00e9thodologie de criblage pour identifier le ph\u00e9notype li\u00e9 \u00e0 des d\u00e9fauts de traduction dans une collection de mutants. Cette collection sera compos\u00e9e de mutants de d\u00e9l\u00e9tion de prot\u00e9ine connue pour interagir avec le ribosome et dont la fonction reste non d\u00e9finie. En parall\u00e8le, nous allons tenter une approche par \u00ab pull-down \u00bb pour identifier de nouveaux facteurs qui interagissent avec les ribosomes bloqu\u00e9s durant la traduction de l’ARNm.<\/p>\n","protected":false},"excerpt":{"rendered":" Th\u00e8me de recherche n\u00b01: Les facteurs de la famille ABC-F La famille ABC-F est une famille de prot\u00e9ines solubles, qui appartient \u00e0 la superfamille des prot\u00e9ines ABC (ATP Binding Cassette). Les prot\u00e9ines qui composent cette superfamille sont des ATPase form\u00e9es de domaines ABC homologues. La plupart des prot\u00e9ines ABC sont des transporteurs membranaires, mais la famille […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":32,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"","ocean_second_sidebar":"","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"on","ocean_display_header":"off","ocean_header_style":"","ocean_center_header_left_menu":"","ocean_custom_header_template":"","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"","ocean_menu_typo_font_family":"","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"Research Themes","ocean_post_subheading":"Physiologie et r\u00e9gulation de la synth\u00e8se prot\u00e9ique","ocean_post_title_style":"background-image","ocean_post_title_background_color":"","ocean_post_title_background":563,"ocean_post_title_bg_image_position":"center center","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"cover","ocean_post_title_height":200,"ocean_post_title_bg_overlay":0.1,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"","footnotes":""},"class_list":["post-1065","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/pages\/1065","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1065"}],"version-history":[{"count":4,"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/pages\/1065\/revisions"}],"predecessor-version":[{"id":1069,"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/pages\/1065\/revisions\/1069"}],"up":[{"embeddable":true,"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/pages\/32"}],"wp:attachment":[{"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1065"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}![\"fig_2\"](\"http:\/\/127.0.0.1:49970\/equipe%20GB\/equipe%20GB\/equipe%20GB\/files\/fig_2.jpg\")
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<\/p>\n\n\n\nTh\u00e8me de recherche n\u00b02:
Identification et caract\u00e9risation fonctionnelle de nouveaux facteurs de traduction putatifs<\/h2>\n\n\n\n
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