{"id":32,"date":"2025-04-14T14:43:55","date_gmt":"2025-04-14T12:43:55","guid":{"rendered":"https:\/\/egm.ibpc.fr\/?page_id=32"},"modified":"2026-04-02T17:32:43","modified_gmt":"2026-04-02T15:32:43","slug":"physiology-and-regulation-of-protein-synthesis","status":"publish","type":"page","link":"https:\/\/egm.ibpc.fr\/?page_id=32","title":{"rendered":"Physiology and regulation of protein synthesis"},"content":{"rendered":"\n
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Our Team<\/h2>\n\n\n\n

\u00a0\u00a0\u00a0\u00a0\u00a0The study of the ribosome and mRNA translation started in the 1960s. Since then, the translation factors, essential to recapitulate the translation process\u00a0in vitro<\/em>, have been identified leading to the development of different in vitro translation systems. Since 2000, the technical revolution of structural biology (x-ray crystallography and cryo-electron microscopy) allows us to have a precise view of the molecular mechanisms involved in protein synthesis catalyzed by the ribosome. Nevertheless, even if we can reproduce mRNA translation in vitro under specific conditions (different from the cellular conditions), the current\u00a0in vitro<\/em>\u00a0translation assays are far from matching the efficiency of\u00a0in vivo<\/em>\u00a0translation. This observation suggests that some of the mechanisms and factors controlling the translation process are yet to be discovered. Our goals are:

1\/ To understand at the molecular and physiological level the mechanism of action of newly identified translation factors belonging to the ABC-F protein family.

2\/ To identify new translation factors as well as factors that couple translation to other mechanisms. Our principal interest is to understand the fundamental aspect of protein synthesis mechanisms and regulations. Furthermore, we try to apply our findings to improve methods for protein synthesis\u00a0in vivo<\/em>\u00a0and\u00a0in vitro<\/em>.

We are currently working with two model organisms: Escherichia coli <\/em>and Synechocystis<\/em><\/p>\n\n\n\n

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Recent Publications<\/strong><\/h2>\n\n\n\n

Journal articles<\/p>

2025<\/p>

Anna Lipo\u0144ska, Laura Monlezun, Isaac Wilkins, Saravuth Ngo, Thomas O\u00efffer, et al.. Base composition at the start of the coding sequence controls the balance between translation initiation and mRNA degradation in E. coli. Nucleic Acids Research<\/i>, 2025, 53 (22), ⟨10.1093\/nar\/gkaf1262⟩<\/a>. ⟨hal-05513161⟩<\/a><\/dd><\/dl>
Daniel P.H. Wong, Kam-Ho Wong, Sunjae Park, Gr\u00e9gory Bo\u00ebl, John Hunt, et al.. OPT: Codon optimize gene sequences for E. coli protein overexpression. Journal of Molecular Biology<\/i>, 2025, 437 (15), pp.168965. ⟨10.1016\/j.jmb.2025.168965⟩<\/a>. ⟨hal-05381659⟩<\/a> \"PDF\"<\/a><\/dd><\/dl><\/div><\/em><\/p>\n\n\n\n

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Our Team \u00a0\u00a0\u00a0\u00a0\u00a0The study of the ribosome and mRNA translation started in the 1960s. Since then, the translation factors, essential to recapitulate the translation process\u00a0in vitro, have been identified leading to the development of different in vitro translation systems. Since 2000, the technical revolution of structural biology (x-ray crystallography and cryo-electron microscopy) allows us to […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":29,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"","ocean_second_sidebar":"","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"off","ocean_header_style":"","ocean_center_header_left_menu":"","ocean_custom_header_template":"","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"","ocean_menu_typo_font_family":"","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"(Head: Gr\u00e9gory Bo\u00ebl)","ocean_post_title_style":"background-image","ocean_post_title_background_color":"","ocean_post_title_background":563,"ocean_post_title_bg_image_position":"center center","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":200,"ocean_post_title_bg_overlay":0.1,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"","footnotes":""},"class_list":["post-32","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/pages\/32","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32"}],"version-history":[{"count":43,"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/pages\/32\/revisions"}],"predecessor-version":[{"id":1450,"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/pages\/32\/revisions\/1450"}],"up":[{"embeddable":true,"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=\/wp\/v2\/pages\/29"}],"wp:attachment":[{"href":"https:\/\/egm.ibpc.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}